mouse small intestine Search Results


mouse small intestine  (Trevigen)
93
Trevigen mouse small intestine
Mouse Small Intestine, supplied by Trevigen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse small intestine cell line ice-1  (InSCREENeX gmbh)
90
InSCREENeX gmbh mouse small intestine cell line ice-1
Mouse Small Intestine Cell Line Ice 1, supplied by InSCREENeX gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse small intestine cell line ice-1 - by Bioz Stars, 2026-04
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mouse normal tissue small intestine cdna  (Cosmo Bio USA)
90
Cosmo Bio USA mouse normal tissue small intestine cdna
Mouse Normal Tissue Small Intestine Cdna, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse normal tissue small intestine cdna - by Bioz Stars, 2026-04
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primary mouse small intestine epithelial cells  (Cell Biologics Inc)
90
Cell Biologics Inc primary mouse small intestine epithelial cells
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Primary Mouse Small Intestine Epithelial Cells, supplied by Cell Biologics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary mouse small intestine epithelial cells - by Bioz Stars, 2026-04
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cryopreserved s9 fractions of the icr mouse small intestine and liver  (Sekisui XenoTech)
90
Sekisui XenoTech cryopreserved s9 fractions of the icr mouse small intestine and liver
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Cryopreserved S9 Fractions Of The Icr Mouse Small Intestine And Liver, supplied by Sekisui XenoTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mouse small intestine epithelial cells  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc mouse small intestine epithelial cells
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Mouse Small Intestine Epithelial Cells, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse small intestine epithelial cells - by Bioz Stars, 2026-04
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mitotic activity of the epithelium of the mouse small intestine  (Chrom Tech)
90
Chrom Tech mitotic activity of the epithelium of the mouse small intestine
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Mitotic Activity Of The Epithelium Of The Mouse Small Intestine, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mitotic activity of the epithelium of the mouse small intestine - by Bioz Stars, 2026-04
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mouse small intestinal stem cells  (Jackson Laboratory)
90
Jackson Laboratory mouse small intestinal stem cells
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Mouse Small Intestinal Stem Cells, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse small intestinal stem cells/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
mouse small intestinal stem cells - by Bioz Stars, 2026-04
90/100 stars
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mouse small intestine  (Sekisui XenoTech)
90
Sekisui XenoTech mouse small intestine
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Mouse Small Intestine, supplied by Sekisui XenoTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse small intestine/product/Sekisui XenoTech
Average 90 stars, based on 1 article reviews
mouse small intestine - by Bioz Stars, 2026-04
90/100 stars
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mouse small intestinal epithelial cells (miecs; cp-m037)  (Procell Inc)
90
Procell Inc mouse small intestinal epithelial cells (miecs; cp-m037)
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Mouse Small Intestinal Epithelial Cells (Miecs; Cp M037), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse small intestinal epithelial cells (miecs; cp-m037)/product/Procell Inc
Average 90 stars, based on 1 article reviews
mouse small intestinal epithelial cells (miecs; cp-m037) - by Bioz Stars, 2026-04
90/100 stars
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genomic dnas of mouse small intestine  (BioChain Institute)
90
BioChain Institute genomic dnas of mouse small intestine
( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal <t>epithelial</t> cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).
Genomic Dnas Of Mouse Small Intestine, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
genomic dnas of mouse small intestine - by Bioz Stars, 2026-04
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mouse small intestine smooth muscle primary cells complete growth medium  (Innovative Research Inc)
N/A
Mouse Small Intestine Smooth Muscle Primary Cells Complete Growth Medium is a primary cells complete growth medium from Innovative Research, supplied as a ready-to-use liquid. More Details: Bacterial detection: Negative Fungal detection: Negative Mycoplasma detection:
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Image Search Results


( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal epithelial cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).

Journal: Nature Communications

Article Title: Circadian control of bile acid synthesis by a KLF15- Fgf15 axis

doi: 10.1038/ncomms8231

Figure Lengend Snippet: ( a ) In situ hybridization analysis of Klf15 and Fgf15 expression in Klf15 +/+ and Klf15 −/− mouse ileum. White arrows indicate Klf15 or Fgf15 mRNA signal. ( b ) Effects of adenoviral overexpression and shRNA knockdown of Klf15 on Fgf15 mRNA expression in primary mouse small intestinal epithelial cells. Primary small intestinal epithelial cells were infected with control virus ( Ctl ), ad Klf15 , or sh Klf15 as indicated. ( c ) Fgf15-Luc reporter assay in Caco2 cells following heterologous overexpression of Flag-Klf15 ( Klf15 ) or pc-DNA3.1 (control, Ctl ). ( d ) ChIP analysis of KLF15 ( Flag-Klf15 ) binding to designate regions of the Fgf15 and the Kv channel-interacting protein 2 ( KChip2 ) promoters in the mouse colon cell line CT26. The relative binding abundance (Rel abundance) was normalized to IgG. ( e ) Fgf15 mRNA expression in Klf15 +/+ versus Klf15 −/− mouse intestine segments: Duo, Jej, ileum and colon ( n =8). ( f ) Circadian rhythm of Klf15 mRNA expression in Klf15 +/+ mouse ileum ( P <0.01) was abolished in Klf15 −/− mouse ileum ( n =5 per time point). ( g ) Immunoblot of circadian KLF15 protein expression in the ileum (representative of three experiments). Ileal Fgf15 mRNA expression ( h ) and blood FGF15 protein concentrations (Conc) ( i ) exhibit circadian variations in Klf15 +/+ mice ( P <0.01) but the rhythms in Klf15 −/− mice were lost with increased expression at indicated time points ( n =5). Data represent mean±s.e.m. Statistical significance of circadian rhythm for each genotype was analysed using analysis of variance followed by Bonferroni post-test. Statistical significance between the two individual groups was assessed using Student's t- test. * indicates P <0.05, compared with control ( Ctl ), or Klf15 +/+ mice. Scale bars, 100 μm ( a ).

Article Snippet: Primary mouse small intestine epithelial cells were purchased from Cell Biologics Inc. (Chicago, IL, USA) and cultured in epithelial cell medium containing 0.1% epidermal growth factor, 0.1% insulin-transferrin sodium selenite, 0.1% hydrocortisone, 5% FBS, 1% L-glutamine and a 1% antibiotic-antimycotic solution on gelatin (Sigma-Aldrich, St Louis, MO, USA)-coated plates.

Techniques: In Situ Hybridization, Expressing, Over Expression, shRNA, Knockdown, Infection, Control, Virus, Reporter Assay, Binding Assay, Western Blot